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To set up and run Space Ranger, follow these steps:
Space Ranger 3.1.1 (Sep 9, 2024)
- Visium Spatial Software Suite
- Self-contained, relocatable tar file. Does not require centralized installation.
- Contains binaries pre-compiled for CentOS/RedHat 7.0 and Ubuntu 16.04.
- Runs on Linux systems that meets the minimum compute requirements.
tar.gz compression
Download for Linux 64-bit (tar.gz)File size: 827 MBmd5sum: c0b9a274d7d3692e28f086818eb55e62curlwgetcurl -o spaceranger-3.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.1.tar.gz?Expires=1732259235&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=TYSnXoexJ810yjEHgBubWxHhseq~E1kiH-k99cViLDuHqBrvqM4DFpaE1sg7Br5KcfhnI2lAlTuvdcquA3nkOp5s5srwX5SRPkkk6685ZiH3MEmh2ZtIHb8K52JuTfcobrwqTR-ggPnHJ7Y57YE2iEy6fTYoqBhqyDq-bAIevD5LKJkMl4fQe4ySlVxdIf6-PfkiLFinXCR-X30YFR-6bDe7~XUjWIiesNhc8sw6nW~GZHqsPHDtEDsczxNLhS2PjZb-BafOvLoBR3Wse-YpkTR~jhvT9gvpHdRdAyGwlqhY6J6BCdqoPOJUoWVMa0EtOI0XsKRcXTKoXvqY9cBgMg__"
tar.xz compression
Download for Linux 64-bit (tar.xz)File size: 527 MBmd5sum: 4adde78374877cee92d056b903a77759curlwgetcurl -o spaceranger-3.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.1.tar.xz?Expires=1732259235&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=JEP7l8VK4AFe4EQ7veAhY-asG5ASjNlD0-HY7KZizUUrBEVjeyd1DZWzL-IrUzUvAZsovUiJn-O5myEi7WMfpvoap9mIv3orlnvCmTdxmY4s7ZFsgod6wyqBwdS9hGj3RBJkMl8gz~KpOsv-itXjDuiipGM4Ja8sI87HDRAglYThWrxbPjQHpYf9ppGITPN91cuEmM42gZfWP0HxP9m5NuA6IGWFPOs97TU9fW70RHBAhEa7yYghPSfkm7PJJEk509REgWkCAdqiXjJG-I1DRFl06yPlM30qd7rjWjSoV6zPnGwsZ3inzCCz6UxceK83SlvcKsy-nUlnH1y-~itL~w__"
Download is unavailable on mobile devices.
Space Ranger v3.1.1 highlights
- In Space Ranger v3.1.1, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.
References
References are required to run the spaceranger count
pipeline. 10x Genomics offers pre-built human and mouse references for download. You can alternatively create a custom reference.
Human reference (GRCh38)
- Human reference (GRCh38)
Download Human ReferenceFile size: 11 GBmd5sum: dfd654de39bff23917471e7fcc7a00cdcurlwgetcurl -O "https://cf.10xgenomics.com/supp/spatial-exp/refdata-gex-GRCh38-2020-A.tar.gz"
mm10 Reference - 2020-A (June 23, 2020)
- Mouse reference (mm10)
Download Mouse ReferenceFile size: 9.7 GBmd5sum: 886eeddde8731ffb58552d0bb81f533dcurlwgetcurl -O "https://cf.10xgenomics.com/supp/spatial-exp/refdata-gex-mm10-2020-A.tar.gz"
Download is unavailable on mobile devices.
Probe Sets
Visium probe-based assays target genes in the human or mouse transcriptomes. For these assays, it is required to specify the relevant probe set to the View more details about probe setsspaceranger count
pipeline. Probe sets come bundled with the Space Ranger package and are also available for download here for convenience.
Human Transcriptome v2
- Probe Set CSV v2
Input for spaceranger count pipeline, CytAssist compatible
- Probe Set BED v2
- Possible Off-Target Probes CSV v2
- Probe Set Metadata TSV v2
- Probe Set Metadata Excel Spreadsheet v2
Human Transcriptome v1
- Probe Set CSV v1
Input for spaceranger count pipeline
- Probe Set BED v1
- Possible Off-Target Probes CSV v1
- Probe Set Metadata TSV v1
- Probe Set Metadata Excel Spreadsheet v1
Mouse Transcriptome v2
- Probe Set CSV v2
Input for spaceranger count pipeline, CytAssist compatible
- Probe Set BED v2
- Possible Off-Target Probes CSV v2
- Probe Set Metadata TSV v2
- Probe Set Metadata Excel Spreadsheet v2
Mouse Transcriptome v1
- Probe Set CSV v1
Input for spaceranger count pipeline, CytAssist compatible
- Probe Set BED v1
- Possible Off-Target Probes CSV v1
- Probe Set Metadata TSV v1
- Probe Set Metadata Excel Spreadsheet v1
Slide File Download
If Space Ranger is to be run in an environment without access to the Internet, the pipeline will require a Visium slide layout file using the --slidefile
option. Enter the slide serial number below to download the corresponding slide layout file.
Other downloads
Feature Reference CSV Files
System requirements
View the minimum requirements to run Space Ranger pipelines
Installation
View instructions on how to install Space Ranger