This Technical Note provides an overview of the Cell Segmentation Staining workflow as well as data highlights.

Xenium In Situ Multimodal Cell Segmentation: Workflow and Data Highlights

Prepare tissue sections on Xenium slides for on-instrument analysis by following our step-by-step User Guide/Protocols.

Assay

The Xenium In Situ enables high-throughput subcellular mapping of 100s of RNA targets, revealing new insights into cellular structure and function. The platform includes a versatile and easy-to-use instrument, sensitive and specific chemistry, and a diverse menu of customizable panels.

Plan ahead by referring to the list of equipment, kits, and reagents needed for the Xenium Gene Expression.

Experimental Design

Experimental Design & Planning

Learn about the pre-designed Gene Expression panels and engage with 10x design experts to design, order, and manufacture Xenium Custom Genes.

Panel Design

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for FFPE.

Tissue Prep for FFPE

For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Demonstrated Protocols for Fresh Frozen.

Tissue Prep for FF

Tissue Prep for Fresh Frozen

Refer to instrument-specific documents for seamless instrument operation.

Instruments

Analyze your Xenium in situ data using our pipelines and visualization tools.

Software Analysis

Safety Data Sheets

Xenium Analyzer

In Situ Gene Expression

Overview

Documentation

Datasets

Videos

Service Providers

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What is In Situ Gene Expression?

Find resources, from protocols to free-to-use analysis tools. Workflow varies based on your sample type. Xenium In Situ Gene Expression currently supports both fresh frozen and FFPE samples.

Access free, publicly available data from a number of different sample types generated with Xenium In Situ solutions.

Xenium In Situ Tissue Preparation

Xenium In Situ Assay Protocols - Best Practices

Find answers to common technical questions, from sample prep through data analysis, about In Situ workflows. 

Xenium In Situ Multimodal Cell Segmentation: Workflow and Data Highlights

Technical Note, CG000750

CG000750_XeniumInSitu_CellSegmentation_TechNote_RevA.pdf