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Easy to use, no single cell sequencing expertise required.
Save time and precious samples
Take the guesswork out of optimizing tissue dissociation and nuclei isolation.
Be confident in your results
Reliable, standardized reagents designed for 10x Genomics single cell assays.
Explore what you can do
Resolve true tissue composition
Characterize the heterogeneity of tissues more completely by preserving cells often lost during fresh tissue dissociation. Leverage streamlined nuclei isolation to avoid long enzymatic incubations that can alter gene expression and create cell-type biases.
Reveal gene regulation mechanisms
Use nuclei to capture gene expression and chromatin accessibility information to explore gene regulatory interactions driving cell differentiation, development, and disease.
Preserve the biological state of your sample
Freeze your sample in time and preserve the biological state. Transport your samples for sample preparation at a central facility or store them for a later date, minimizing changes in gene expression.
Expand your reach to stored samples
Reevaluate biobanked frozen tissues using new single cell technologies, and address scientific questions historically left unanswered.
Proven Results
Resources
Find the latest app notes and other documentation on the Chromium Single Cell Nuclei Isolation Kit.
Sample preparation for single cell applications in oncology
Sample preparation for single cell applications in oncology
Sample Preparation Guide, 10x Genomics
Sample Preparation Guide, 10x Genomics
Sample preparation optimization guide with Single Cell Gene Expression LT
Sample preparation optimization guide with Single Cell Gene Expression LT
Sample Preparation Guide, 10x Genomics
Sample Preparation Guide, 10x Genomics
Multiply your power of discovery, simplify your workflows
Multiply your power of discovery, simplify your workflows
Multiome/Nuclei Isolation Flyer, 10x Genomics
Multiome/Nuclei Isolation Flyer, 10x Genomics
Our end-to-end solution
Nuclei preparation
All-in-one kit uses standard laboratory equipment, no special instrumentation needed.
Select your single cell assay
Nuclei can be used to capture chromatin accessibility or whole transcriptome gene expression profiles.
Intuitive analysis and visualization software
Discover single cell analysis, including cloud data processing, Cell Ranger analysis pipelines, and Loupe, our visualization app.
World-class technical and customer support
Our expert support team can be contacted by phone or email.
Workflow
Scroll down to see the base workflow for Chromium Nuclei Isolation.
- 1
Obtain your sample
Start with your frozen tissue of interest. Compatible sample types include mouse heart, liver, brain, and a variety of human tumors.
Resources - 2
Break apart your frozen sample
Use the provided pestle and lysis buffer to mechanically disrupt the frozen tissue into a homogeneous mix.
Resources - 3
Isolate nuclei
Pass the homogenized tissue through the provided spin column to isolate nuclei from the disrupted sample.
- 4
Remove debris
Clean up the nuclei suspension using the supplied debris removal solution and buffer washes.
- 5
Resuspend nuclei and QC
Resuspend the nuclei in the recommended resuspension buffer, check nuclei quality, and proceed to your single cell assay of choice.
Resources
Frequently asked questions
The Chromium Nuclei Isolation Kit has been validated with the following frozen tissues: mouse heart, liver, brain, lung, kidney, small intestine, and a variety of human tumors. An up-to-date list of tissues tested is available on the 10x Genomics Support site. This kit is not compatible with cell suspensions, plant or insect material, calcified tissues, or FFPE tissues.
Supported tissue sizes range from 3-50 mg per column. For pieces of tissue which are larger, break up the tissue into 50 mg pieces, run multiple dissociation columns, and combine nuclei at the final step. It may be possible to run pieces <3 mg, but yield is dependent on tissue type.
All the consumables and reagents needed to generate a clean nuclei suspension are included in the kit and utilize standard lab equipment. No specialized instrument is needed.
Compatible single cell assays include Chromium Single Cell ATAC, Single Cell Multiome ATAC + Gene Expression, Single Cell Gene Expression, and Single Cell Gene Expression Flex. Follow the nuclei resuspension recommendations for the specific assay you are running. No modifications to the single cell workflows are necessary.
10x Genomics provides analysis and visualization software to help you analyze your single cell data. Follow analysis recommendations for the single cell assay of interest. For single cell gene expression assays, make sure introns are included during analysis. You can download Cell Ranger and Loupe Browser software from the 10x Genomics Support site at no cost.
